RöFo_2013_05-MRT-US-Echtzeitfusionsbiopsie der Prostata
Abstracts zum Kongress der Deutschen Gesellschaft für Urologie. Download PDF. Transrektalen Prostatabiopsie Technik Documents. Jung1, K. Schmitt2, M. Saar1, K. Junker1, M. Stöckle1, G. Wagenlehner1, P. Weidner1, K. Steger1, S. Hat nicht der Erstautor den Vortrag gehalten, so ist der Referent in der Autorenzeile unterstrichen. Stromal cells mediate normal prostate development and a contribution to prostate cancer Pca is well documented, but their tissue specific expression profile is imperfect.
Thus, a comprehensive and comparative analysis of fibroblasts from tumor-specimens and BPH derived fibroblasts is indispensable to understand their cancer-specific function. Material and methods. CGH analysis revealed normal karyotypes without any chromosomal alterations in all fibroblast cell cultures tested.
Thus, these results provide new aspects concerning the participation and efficacy of stromal cells in PCa. Saar1, M.
Even though there has been much effort to culture patient derived prostate cancer cells in serial passage, in vitro approaches usually do not represent the heterogeneity of this tumor and therefore mostly fail. Thus we used a new 3D suspension spheroid system to establish cell cultures from transrektalen Prostatabiopsie Technik prostatectomy specimen in serial passage. Tissue from confirmed cancer areas of high risk prostatectomy transrektalen Prostatabiopsie Technik was used to extract cell clusters by limited digestion.
Spheroids were cultured in ultralow plates using suspension culture and passaged via trypsin digestion. Cultures were transrektalen Prostatabiopsie Technik date partly preserved until passage 4.
Spheroids proofed viability for months with Ki67 expression and prostate marker positivity for CK5 and CK8 as well as androgen receptor. PSA levels were high in culture medium suggesting a secretory function of these spheroids. In vitro culture of primary tissue using suspension culture techniques seems to be very promising. Further characterization steps are necessary to evaluate this model in vitro and transfer this approach to other prostate cancer stages.
A comprehensive othtotopic in vivo model will confirm the in vitro results and offer new perspectives in translational prostate cancer research.
Saar1, J. Jung1, A. Unteregger1, M. Even though prostate cancer PC researchers strongly demand for better in vivo models, orthotopic models that display intraprostatic tumor growth and progression have not been established so far.
Tumor tissue could serve as a relevant source, but this requires new techniques and skills. Tissue from high risk PC patients was grafted orthotopically in nude mice.
While testosterone propionate was supplemented to support tumor engraftment, animals were monitored by high-resolution ultrasound and serum PSA-measurements.
Autopsy and histology served to confirm orthotopic growth, whilst immunostaining of paternal and animal tumors as well as PCR for human tissue and androgen deprivation were transrektalen Prostatabiopsie Technik to further characterize this preclinical model. A first series showed an overall engraftment rate of Ultrasound detected tumor foci and documented tumor growth while these animals were selectively PSA positive 0.
Ki indices ranged from 4— In serially implanted animals androgen deprivation caused a rapid decline in PSA transrektalen Prostatabiopsie Technik immunhistochemistry after castration transrektalen Prostatabiopsie Technik under investigation. Orthotopic tumorgrafts retain histological properties of the parental tumor and allow in vivo follow up via serum PSA and threedimensional ultrasound.
This is a promising approach for an optimized in vivo model to investigate transrektalen Prostatabiopsie Technik initiation, stromal interactions and metastatic progression. Zengerling1, J. Steinestel1, M. Schrader1, F. Jentzmik1, A. Schrader1, M.
Castration resistant prostate cancer is characterized by an increase of C-terminally truncated, constitutively active androgen receptor AR variants. In vivo, BPAF activity was analyzed in a chick chorioallantoic membrane xenograft assay. AR and PSA protein-levels were determined by immunohistochemistry. Based on previous observations transrektalen Prostatabiopsie Technik is transrektalen Prostatabiopsie Technik that AR and QX are able to synergistically activate the PSA promoter in absence of androgens.
Burchardt1, M. Abiraterone is an inhibitor of cytochrome P 17A1 and a promising drug for castration resistant prostate cancer PCa therapy. In order to better transrektalen Prostatabiopsie Technik the mode of action of transrektalen Prostatabiopsie Technik and to benchmark therapy sequences of next-generation PCa drugs, basic molecular characterizations of abiraterone effects in PCa cell lines have been conducted.
Materials and methods. Most notably, PC-3 cells responded to abiraterone treatment as well, although being characterized as AR negative. Furthermore, apoptotic factors were upregulated p53, HSP60 and in contrast to other cytostatic drugs, Abstracts no induction of the cytoprotective factor HSP27 was detectable. Besides abiraterone mediated inhibition of steroid synthesis, this study represents evidence for steroid-independent pathways blocked by abiraterone, as seen in decreased proliferation rates of PC-3 cells.
Abiraterone efficacy may be mediated by destabilization of AR turn-over by heat shock protein suppression, next to the induction of apoptotic pathways. HSPmediated transrektalen Prostatabiopsie Technik mechanisms have not been identified. Stope1, M. Streitbörger1, U. Zimmermann1, R. With next-generation prostate cancer PCa drugs new treatment options are available. However, advanced PCa therapies appeared restricted by primary and secondary resistances as well as by mechanisms of cross resistance.
In this study a chemotherapy transrektalen Prostatabiopsie Technik system was established utilizing docetaxel treated PCa cell lines for analysis of molecular resistance machineries.
Both, overexpression of a phosphorylation mimicking HSP27 mutant and the induction of HSP27 phosphorylation specific kinase activators have shown that phosphorylation of HSP27 is the initial step in induction of docetaxel resistance. Taken together, our data point at a chemotherapy-induced and HSP27 — miR-1 mediated resistance loop restoring miR-1 blocked cellular growth, subsequently, counter-acting cytostatic effects of docetaxel.
Beyond that, HSPspecific kinase activators and phosphatase inhibitors as well as miR-1 mimicking nucleic acid therapeutics could facilitate HSP27 inhibition therapy and may prevent chemoresistance.
Hampel2, F. Roos2, J. Thüroff2, W. Brenner2 1 Fukushima Medical University, Dept. Although TKI treatment shows promising results, often resistance develops. Here we investigated the molecular background of this effect.
Six human renal cell carcinoma cell lines were treated with Sorafenib, Sunitinib and Pazopanib at concentrations of 0. These effects were prevented by using Tocilizumab.
Our results indicate transrektalen Prostatabiopsie Technik the enhanced cellular activity after TKI treatment in concentrations under the therapeutical level is transrektalen Prostatabiopsie Technik by a high IL-6 secretion in RCC cells.
Since Tocilizumab prevents this effect, IL-6 could be a possible target for overcoming the development of resistance to TKI. Unteregger1, J. Stöckle1, K. Treatment of advanced renal cell carcinoma RCC by tyrosine kinase inhibitors like Sunitinib SUN is hampered by the appearance of drug resistance. Microarray analysis was performed for a global miRNA expression profiling. Resistance-development as well as drug efficacy during sequence therapy was monitored by sequential quantification of the growth behavior and changes in drug-sensitivity by proliferation-assays.
According to this phenomenon we observed characteristic changes in miRNA expression. The results indicate a complex mechanism in drug resistance depending on therapeutic protocol and cell type. However, drugresistant cell lines offer a promising tool to elucidate the molecular mechanism responsible for drug-resistance as well transrektalen Prostatabiopsie Technik to simulate the benefit of a sequence therapy. Since miRNAs represent an important tool in gene regulation the observed patterns may help to elucidate new genes involved in drug resistance in ccRCC.
V 02 — Operative Therapie von Blasenfunktionsstörungen Al-Mansur1, M. Implantate zur Beckenbodenrekonstruktion senken zwar die Rezidivrate können aber Komplikationen und einen negativen Einfluss auf die Lebensqualität LQ haben.
Material und Methoden. Keine 16 Pat. Keine 3 Pat. Insgesamt wurden 18 vordere Netze, 11 hintere Netze und 67 totale Netze verwandt.
Hierüber wurde ein gesamter Beckenbodendysfunktionsscore erhoben max. Bisherige Therapien bestehen daher primär in der Therapie der Symptome ohne die Ursache zu behandeln. Transrektalen Prostatabiopsie Technik und Methode.